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11.
移动IP技术在VPN中的应用 总被引:2,自引:0,他引:2
虚拟专用网络VPN(Virtual Private Networks)是实现在公网上安全传输私有网络信息的一种技术.作为VPN的关键技术之一的隧道技术解决了移动节点的移动性问题.在介绍L2TP与IPSec这2种VPN隧道机制的基础上对它们进行了比较,重点介绍了IPSec在安全性方面的优势,然后介绍了VPN移动用户的问题和VPN的2种隧道模型,最后分析了利用移动IP技术解决VPN中的移动用户问题的优点,并与现有方案VPDN(Virtual Private Dial-up Network)作了比较. 相似文献
12.
芦荟中蒽醌类化合物成分研究 总被引:10,自引:0,他引:10
利用聚酰胺、硅胶柱色谱,结合溶剂分配及重结晶技术,从芦荟叶液汁的浓缩物提取物中分离得到6种蒽醌类化合物,经综合波谱分析和化学方法鉴定它们的结构,结果为芦荟大黄素(aloeemodin)、大黄素甲醚(physi cion)、大黄酚 (chrysophanol)、大黄素 (emodin)、大黄酸 (rhein)及一个自然界新发现的化合物:1,8-二羟基-9,10-蒽酮3-甲基-(2羟基)丙酸酯 (1,8-dihydroxy-9,10-anthraquinone-3-methyl-(2-hydroxy)propionateester)。 相似文献
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14.
The Wnt/beta-catenin/TCF4 pathway plays critical roles in the maintenance of small intestinal epithelium; however, downstream
targets of the beta-catenin/TCF4 complex are not extensively characterized. We identified miR-30e as an immediate target activated
by the beta-catenin/TCF4 complex. miR-30e was detected in the peri-nuclear region of the intestinal crypt IEC-6 cells. Bioinformatics
analysis revealed clustered beta-catenin/TCF4 binding sites within the miR-30e promoter region. This promoter region was cloned
into pGL3-control luciferase reporter vector, with the enhancer region removed. Transfection of pCMV-SPORT6-beta-catenin expression
vector dose-dependently increased luciferase activity, and co-transfection of pCMV-SPORT6-TCF4 expression vector further enhanced
the promoter activity. Dexamethasone-induced IEC-6 cells differentiation caused a 2.5-fold increase in miR-30e expression,
and upon beta-catenin siRNA transfection, miR-30e increased 1.3-fold. Electrophoretic mobility shift assay and chromatin immunoprecipitation
assay confirmed the binding between beta-catenin/TCF4 complexes from IEC-6 nuclear extracts and the putative sequences in
the miR-30e promoter. These results demonstrate that beta-catenin/TCF4 transactivates miR-30e during intestinal cell differentiation. 相似文献
15.
16.
A radiation hybrid map of the rat genome containing 5,255 markers. 总被引:17,自引:0,他引:17
T K Watanabe M T Bihoreau L C McCarthy S L Kiguwa H Hishigaki A Tsuji J Browne Y Yamasaki A Mizoguchi-Miyakita K Oga T Ono S Okuno N Kanemoto E Takahashi K Tomita H Hayashi M Adachi C Webber M Davis S Kiel C Knights A Smith R Critcher J Miller T Thangarajah P J Day J R Hudson Y Irie T Takagi Y Nakamura P N Goodfellow G M Lathrop A Tanigami M R James 《Nature genetics》1999,22(1):27-36
A whole-genome radiation hybrid (RH) panel was used to construct a high-resolution map of the rat genome based on microsatellite and gene markers. These include 3,019 new microsatellite markers described here for the first time and 1,714 microsatellite markers with known genetic locations, allowing comparison and integration of maps from different sources. A robust RH framework map containing 1,030 positions ordered with odds of at least 1,000:1 has been defined as a tool for mapping these markers, and for future RH mapping in the rat. More than 500 genes which have been mapped in mouse and/or human were localized with respect to the rat RH framework, allowing the construction of detailed rat-mouse and rat-human comparative maps and illustrating the power of the RH approach for comparative mapping. 相似文献
17.
c-Abl, a non-receptor tyrosine kinase, is activated by agents that damage DNA. This activation results in either arrest of the cell cycle in phase G1 or apoptotic cell death, both of which are dependent on the kinase activity of c-Abl. p73, a member of the p53 family of tumour-suppressor proteins, can also induce apoptosis. Here we show that the apoptotic activity of p73alpha requires the presence of functional, kinase-competent c-Abl. Furthermore, p73 and c-Abl can associate with each other, andthis binding is mediated by a PxxP motif in p73 and the SH3 domain of c-Abl. We find that p73 is a substrate of the c-Abl kinase and that the ability of c-Abl to phosphorylate p73 is markedly increased by gamma-irradiation. Moreover, p73 is phosphorylated in vivo in response to ionizing radiation. These findings define a pro-apoptotic signalling pathway involving p73 and c-Abl. 相似文献
18.
从力学原理、设计原理、整机结构、关键零部件的设计和工作程序,系统地介绍了自调式镦压挤胀复合液压机.由于压机设计了顶出缸对下活动横梁调节限位结构,回程拉杆对上镦压横梁的复位结构及镦压缸和气液储能器之间的连通协调结构,这不仅使该液压机结构紧凑,同时节省了上镦压横梁的回程液压缸、下活动横梁的镦压缸,并简化了上凸模与上镦压模分设的液压系统,而且解决了直齿圆柱齿轮在塑性成形过程中齿顶难以充满、齿根易出现微裂纹,以及成形压力过大和模具寿命过低的问题. 相似文献
19.
Distinct and evolutionarily conserved signal-transduction cascades mediate the survival or death of cells during development. The c-Jun amino-terminal kinases (JNKs) of the mitogen-activated protein kinase superfamily are involved in apoptotic signalling in various cultured cells. However, the role of the JNK pathway in development is less well understood. In Drosophila, Decapentaplegic (Dpp; a homologue of transforming growth factor-beta) and Wingless (Wg; a Wnt homologue) proteins are secretory morphogens that act cooperatively to induce formation of the proximodistal axis of appendages. Here we show that either decreased Dpp signalling in the distal wing cells or increased Dpp signalling in the proximal wing cells causes apoptosis. Inappropriate levels of Dpp signalling lead to aberrant morphogenesis in the respective wing zones, and these apoptotic zones are also determined by the strength of the Wg signal. Our results indicate that distortion of the positional information determined by Dpp and Wg signalling gradients leads to activation of the JNK apoptotic pathway, and the consequent induction of cell death thereby maintains normal morphogenesis. 相似文献
20.
E A Rogaeva S Premkumar J Grubber L Serneels W K Scott T Kawarai Y Song D L Hill S M Abou-Donia E R Martin J J Vance G Yu A Orlacchio Y Pei M Nishimura A Supala B Roberge A M Saunders A D Roses D Schmechel A Crane-Gatherum S Sorbi A Bruni G W Small P M Conneally J L Haines F Van Leuven P H St George-Hyslop L A Farrer M A Pericak-Vance 《Nature genetics》1999,22(1):19-22